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&&是不是彻底没戏了？急！！！求高手指点。
是不是彻底没戏了？急！！！求高手指点。
RT:刚收到意见：编辑意见如下。
With reference to above manuscript, I am sorry to have to tell you that your paper has not been accepted for publication in the Journal of Electroanalytical Chemistry since the referees' reports were not positive.&&The comments of the referees are included below. Evidently, while the referees saw some merit in the paper, they had difficulty in following some arguments due to poor English expression in the manuscript. Should you be able to address this aspect and the other comments, you are welcome to send a new version of the paper, but it would have to be considered as a new submission.
求教，这篇文章还有戏吗？
改语言，再以新稿件投吗？
哪还有接受的可能吗？是不是要换人重新再审，那题目需不需要在改改？题目不改，只改内容和语言，
又得等个把月啊。。。重新提交的话:shuai::shuai::shuai::(:(:(:(
嗯啊，个把月就很短了。。。。。。
“Evidently, while the referees saw some merit in the paper, they had difficulty in following some arguments due to poor English expression in the manuscript. ”根据这句话得出有戏的结论？
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&&PCR高手请进！这个PCR 扩增条件看不懂，求高手指点！急！急！
PCR高手请进！这个PCR 扩增条件看不懂，求高手指点！急！急！
我通过查文献，要扩2对长引物（6kb，9kb），扩增条件是：
92℃变性 2min
92℃变性 10sec
50℃退火 30sec 10 个循环
60℃延伸 10min
92℃变性 2min
92℃变性 10sec
50℃退火 30sec 20 个循环，每个循环加 10sec
60℃延伸 10min
60℃延伸 10min
就是后20个循环，每个循环加10S，在哪里加呀，仪上能分开两部分吗？为什么第一个10个循环后，还要来一个92度预变性2min。
求高手指点，急呀！
再问一下楼上，每个循环加10S是总共加10S，还是说变性，退火，延伸各加10S呢?不知这是什么原理，和总共30个循环有差别吗，
一般只改变退火的条件，在这个例子中就是退火时间加10s，其他不变
touchdown 倒是做过，每个循环降低退火温度。每个循环延长退火时间的，我也没做过。一般来说，退火时间太短可能会导致引物和模板结合不充分，退火时间增加有利于引物和模板退火，但也增加了模板两条链之间退火的概率。
感谢楼上的回复，虽然还不明白是怎么回事:)
学术必备与600万学术达人在线互动！
扫描下载送金币高手指点！急！急！
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