考点：基因工程的原理及技术
分析：根据题中信息，原来质粒长度为3.7kb，切去EF之间的片段（0.2kb）还剩3.5kb，插入目的基因（1.2kb）后构成重组质粒，则重组质粒长度为1.2+3.5=4.7kb．重组质粒插入在一条染色体DNA上，可通过雌雄转基因山羊杂交的方法来获取转基因纯合子山羊．目的基因的模版连中碱基为TGA，则mRNA中对应的密码子为ACU，tRNA与mRNA互补配对，则翻译时识别该密码子的tRNA上相应的碱基序列是UGA．
解：（1）限制酶E和与限制酶F都是蛋白质，它们在结构和功能上的不同之处分别在于：①蛋白质分子结构不同（氨基酸的种类、数量、排序不同，肽链的空间结构不同）；②限制酶具有专一性，因此它们识别的脱氧核苷酸序列不同．（2）根据题中信息，原来质粒长度为3.7kb，切去EF之间的片段（0.2kb）还剩3.5kb，插入目的基因（1.2kb）后构成重组质粒pZHZ2，则重组质粒pZHZ2长度为1.2+3.5=4.7kb．（3）将重组质粒pZHZ2导入至山羊的受精卵细胞中，培养形成转基因山羊．若pZHZ2进入细胞后插入在一条染色体DNA上，则转基因山羊可以看成是杂合子，那么获得转基因纯合子山羊的方式是让转基因山羊间相互交配．（4）目的基因的模版连中碱基为TGA，则mRNA中对应的密码子为ACU，tRNA与mRNA互补配对，则翻译时识别该密码子的tRNA上相应的碱基序列是UGA．故答案为：（1）①蛋白质分子的结构不同（氨基酸的种类、数量、排序不同，肽链的空间结构不同）②识别的脱氧核苷酸序列不同（2）4.7（3）转基因山羊间相互交配（4）UGA
点评：本题结合题图，考查基因工程的相关知识，意在考查考生的识图能力和理解所学知识要点，把握知识间内在联系，形成知识网络结构的能力；能运用所学知识，对生物学问题作出准确判断和得出正确结论的能力．
请选择年级高一高二高三请输入相应的习题集名称(选填)：
科目：高中生物
判定从植物体榨出的液体是否含有脂肪，酵母菌洒精发酵是否彻底，酪蛋白是否充分水解成氨基酸，最好依次选择的试剂（　　）①苏丹Ⅲ②Ca（OH）2&&&&③班氏试剂&&&&④双缩脲试剂．
A、①③④B、①②④C、②③④D、①②③
科目：高中生物
下列有关蛋白质和DNA的提取和分离的说法，正确的是（　　）
A、可用蒸馏水胀破细胞的方法从猪红细胞中提取DNA和蛋白质B、提取DNA时，可用不同浓度的NaC1溶液反复溶解与析出蛋白质C、透析法分离蛋白质的依据是蛋白质不能通过半透膜D、用玻璃棒快速搅拌有利于提取DNA
科目：高中生物
胚胎工程是一项综合性的动物繁育技术，控制哺乳动物的性别对于畜牧业生产有着十分重要的意义．如图是培&育出所需性别的试管牛的示意图．回答下列相关问题：．（1）图中过程①通常采用的是体外受精技术，在这个过程中获能的精子与体外培养至（填时期）的卵母细胞结合形成受精卵，防止多精入卵的两个生理反应是为获得更多的卵细胞需要给良种母牛注射适宜剂量的（2）过程②中受精卵培养发育至时期，再经胚胎移植到代孕母牛的子宫中从而获得后代．供体胚胎在受体子宫能存活的生理基础是（3）动物生物反应器是转基因动物技术中最诱人的部分之一．例如将人的血清白蛋白基因导入半的体内，并使之在乳腺中特异性表达出血清白蛋白，在培育转基因的奶牛的过程中，常选用的受体细胞是导入目的基因的常用方法是．
科目：高中生物
台湾塑化剂事件的爆发，使我们认识到环境激素对人体的危害．有人猜测塑化剂的作用类似于雌激素，可能会引发性早熟．请设计实验探究上述猜测是否正确．（说明：性成熟的早晚可以用初次发情时间为指标）（1）材料和用具：同种雌鼠若干，的饲料（甲），含适量浓度雌性激素的饲料（乙），普通饲料（丙）等．（2）实验目的：探究塑化剂的作用是否类似于雌激素，可引发单位性早熟．（3）实验原理：．（4）实验思路：①将雌鼠3组，编号A、B、C．②A组每天饲喂一定量的饲料甲，B组每天饲喂等量的饲料乙，C组每天饲喂等量的饲料丙．③在相同且适宜的条件下饲养．④．（5）如果猜测正确，则实验结果是：．
科目：高中生物
如图为人体内三大营养物质代谢过程示意图，其中数字表示过程，大写英文字母表示物质（1）图中A、B、C、D四种物质中，能在肝脏内形成的是．（2）人体血液中乳酸量增多时，参与调节内环境稳态的物质主要是．（3）E是和；过程16包含循环，过程16的产物中的氧原子来自反应物中的氧气．（4）图中有ATP生成的过程编号有．（5）丙氨酸是非必需氨基酸，赖氨酸是必需氨基酸，当这两种氨基酸含量不足时，补充它们的途径分别是（填编号）和．（6）糖尿病患者患者有“饮水多”之生理现象，其原因是．
科目：高中生物
目前临床上癌症治疗的方法，一般是手术切除肿瘤，为防止体内残留癌细胞，在手术后进行化疗，某生物兴趣小组的同学从资料上获知：二氯二乙胺能够阻止参与DNA复制的酶与DNA相互作用．他们推测：二氯二乙胺能抑制癌细胞的增殖，能作为一种癌症的化疗药物，并就此问题设计实验进行了探究．实验材料：肝长有肿瘤的小鼠，二氯二乙胺溶液，蒸馏水，生理盐水，含有全部营养物质的细胞培养液，显微镜，血球计数板，试管，吸管等．（1）其中某位同学实验操作如下：（请将实验内容补充完整）①取洁净的培养皿一个，加入适量的培养液，从小鼠肝部切取肿瘤组织，剪碎，并用处理，使其分散开来，置于培养皿中培养．②取洁净的试管5支，加入等量的培养液，编号1、2、3、4、5，并在1-4号试管中加入等量的不同浓度的二氯二乙胺溶液，5号试管中加入．③从培养皿中吸取等量的培养液置于1-5号试管中，振荡后，在冰箱中培养一段时间．④从静置的5支试管中吸取适量的培养液置于血球计数板内，在显微镜下计数，记录数据．（2）请你纠正上面这位同学操作上的错误①；②．（3）另一位同学按照正确的方法进行实验，得到的结果如下表：
二氯二乙胺浓度（mg/ml）
细胞数目（个/mL）
560在该实验中，遵循了实验设计的原则和原则．（4）根据上表中实验结果，你能得出的结论是：．
科目：高中生物
在一定实验条件下，测得某植物光合作用速率与光照强度之间的关系、呼吸作用与氧气浓度之间的关系及光合作用速率与温度之间的关系，如图所示，对该图示解释正确的是（　　）
A、影响图甲中曲线上的A点上下移动的主要外界因素是光照强度B、图乙中的数据需在适宜光照条件下测量C、图丙中，若大棚内温度始终处于37.5℃的恒温，每日光照12h，植物体干重将减少D、用大棚种植该蔬菜时，白天应控制光照为C点对应的光照强度，温度为35℃最佳
科目：高中生物
某线性DNA分子含有5000个碱基对（bp），先用限制性核酸内切酶a完全切割，再把得到的产物用限制性核酸内切酶b完全切割，得到的DNA片段大小如下表．限制性核酸内切酶a和b的识别序列和切割位点如图所示．下列有关叙述错误的是（　　）
a醇切割产物（bp）
b醇再次切割产物（bp）
；800；600；
A、a酶与b酶切断的化学键相同B、限制性核酸内切酶a和b切出的DNA片段不能相互连接C、该DNA分子中a酶能识别的碱基序列有3个D、仅用b酶切割该DNA分子至少可得到三种DNA片段
吴老师30日19点直播线段的垂直平分线的性质
余老师30日20点直播unit5第二课时 Section A欢迎来到圣克鲁斯生物技术! &|&&|&语言EnglishFran?ais日本語中文??Espa?olItalianoDeutschSvenskaPortugus
&&&&&&&&&&&&ZFYVE 抗体
ZFYVE Antibodies
简介 HOVERcruz(TM)一个可以快速识别ZFYVE Antibodies的独特系统。将鼠标悬停在表中的产品名称旁，可看到该产品代表的数据。
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产品名称产品编号抗体亚型抗原决定蔟应用种属应用排名 sc-515049小鼠
IgG1402-479 (h)WB, IP, IF, ELISA人WB, IP, IF, ELISA sc-139117山羊 IgGC-terminal (h)WB, IF, ELISA, , WB, IF, ELISA sc-382063兔 IgG402-479 (h)WB, IP, IF, ELISA, , WB, IP, IF, ELISA sc-135608小鼠
IgG2bN/AWB, IP, ELISA人WB, IP, ELISA sc-165939山羊 IgGInternal (h)WB, IF, ELISA, , WB, IF, ELISA sc-165940山羊 IgGInternal (h)WB, IF, ELISA, , WB, IF, ELISA sc-165941山羊 IgGInternal (h)WB, IF, ELISA, , WB, IF, ELISA sc-101051小鼠
IgG1FL (h)WB, IP, IF, ELISA, WB, IP, IF, ELISA sc-79369山羊 IgGC-terminal (h)WB, IP, IF, ELISA, , , , WB, IP, IF, ELISA sc-367699兔 IgG (h)WB, IP, IF, ELISA, , WB, IP, IF, ELISA sc-79372山羊 IgGN-terminal (h)WB, IF, ELISA, , , , , , WB, IF, ELISA sc-102174兔 IgGN/AWB, IP, ELISA, , , 犬WB, IP, ELISA sc-132148山羊 IgGextracellular (h)WB, IF, ELISA, , WB, IF, ELISA sc-241142山羊 IgGC-terminal (h)WB, IF, ELISA, , , , , , , WB, IF, ELISAZFYVE CRISPR 敲除，HDR和双切口酶质粒包括:产品名称产品编号种属应用标记物应用 sc-407076人Gene KnockoutGFPGene Knockout sc-407076-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-407076-NIC人Gene KnockoutPuromycinGene Knockout sc-407076-NIC-2人Gene KnockoutPuromycinGene Knockout sc-407267人Gene KnockoutGFPGene Knockout sc-407267-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-407267-NIC人Gene KnockoutPuromycinGene Knockout sc-407267-NIC-2人Gene KnockoutPuromycinGene Knockout sc-409854人Gene KnockoutGFPGene Knockout sc-409854-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-409854-NIC人Gene KnockoutPuromycinGene Knockout sc-409854-NIC-2人Gene KnockoutPuromycinGene Knockout sc-411338人Gene KnockoutGFPGene Knockout sc-411338-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-411338-NIC人Gene KnockoutPuromycinGene Knockout sc-411338-NIC-2人Gene KnockoutPuromycinGene Knockout sc-412894人Gene KnockoutGFPGene Knockout sc-412894-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-412894-NIC人Gene KnockoutPuromycinGene Knockout sc-412894-NIC-2人Gene KnockoutPuromycinGene Knockout sc-413119人Gene KnockoutGFPGene Knockout sc-413119-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-413119-NIC人Gene KnockoutPuromycinGene Knockout sc-413119-NIC-2人Gene KnockoutPuromycinGene Knockout sc-413741人Gene KnockoutGFPGene Knockout sc-413741-HDR人Homology Directed RepairPuromycinHomology Directed Repair sc-413741-NIC人Gene KnockoutPuromycinGene Knockout sc-413741-NIC-2人Gene KnockoutPuromycinGene Knockout sc-427082小鼠 Gene KnockoutGFPGene Knockout sc-427082-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-427082-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-427082-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-428191小鼠 Gene KnockoutGFPGene Knockout sc-428191-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-428191-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-428191-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-431772小鼠 Gene KnockoutGFPGene Knockout sc-431772-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-431772-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-431772-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-432238小鼠 Gene KnockoutGFPGene Knockout sc-432238-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-432238-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-432238-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-432300小鼠 Gene KnockoutGFPGene Knockout sc-432300-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-432300-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-432300-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-433038小鼠 Gene KnockoutGFPGene Knockout sc-433038-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-433038-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-433038-NIC-2小鼠 Gene KnockoutPuromycinGene Knockout sc-435660小鼠 Gene KnockoutGFPGene Knockout sc-435660-HDR小鼠 Homology Directed RepairPuromycinHomology Directed Repair sc-435660-NIC小鼠 Gene KnockoutPuromycinGene Knockout sc-435660-NIC-2小鼠 Gene KnockoutPuromycinGene KnockoutZFYVE CRISPR激活质粒包括:产品名称产品编号种属应用标记物应用 sc-407076-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-407076-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-407076-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-407076-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-407267-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-407267-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-407267-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-407267-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-409854-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-409854-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-409854-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-409854-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-411338-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-411338-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-411338-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-411338-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-412894-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-412894-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-412894-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-412894-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-413119-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-413119-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-413119-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-413119-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-413741-ACT人Gene ActivationZeo, Blast, HygroGene Activation sc-413741-ACT-2人Gene ActivationZeo, Blast, HygroGene Activation sc-413741-LAC人Gene ActivationZeo, Blast, HygroGene Activation sc-413741-LAC-2人Gene ActivationZeo, Blast, HygroGene Activation sc-427082-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-427082-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-427082-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-427082-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-428191-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-428191-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-428191-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-428191-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-431772-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-431772-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-431772-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-431772-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432238-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432238-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432238-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432238-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432300-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432300-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432300-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-432300-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-433038-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-433038-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-433038-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-433038-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-435660-ACT小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-435660-ACT-2小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-435660-LAC小鼠 Gene ActivationZeo, Blast, HygroGene Activation sc-435660-LAC-2小鼠 Gene ActivationZeo, Blast, HygroGene ActivationZFYVE 特异 siRNA, shRNA 质粒和 shRNA 慢病毒颗粒基因沉默子产品包括：产品名称产品编号种属应用标记物应用 sc-63243人Gene SilencingN/AGene Silencing sc-63243-SH人Gene SilencingPuromycinGene Silencing sc-63243-V人Gene SilencingPuromycinGene Silencing sc-63244小鼠 Gene SilencingN/AGene Silencing sc-63244-SH小鼠 Gene SilencingPuromycinGene Silencing sc-63244-V小鼠 Gene SilencingPuromycinGene Silencing sc-89030人Gene SilencingN/AGene Silencing sc-89030-SH人Gene SilencingPuromycinGene Silencing sc-89030-V人Gene SilencingPuromycinGene Silencing sc-90097人Gene SilencingN/AGene Silencing sc-90097-SH人Gene SilencingPuromycinGene Silencing sc-90097-V人Gene SilencingPuromycinGene Silencing sc-90825人Gene SilencingN/AGene Silencing sc-90825-SH人Gene SilencingPuromycinGene Silencing sc-90825-V人Gene SilencingPuromycinGene Silencing sc-91838人Gene SilencingN/AGene Silencing sc-91838-SH人Gene SilencingPuromycinGene Silencing sc-91838-V人Gene SilencingPuromycinGene Silencing sc-92096人Gene SilencingN/AGene Silencing sc-92096-SH人Gene SilencingPuromycinGene Silencing sc-92096-V人Gene SilencingPuromycinGene Silencing sc-92444人Gene SilencingN/AGene Silencing sc-92444-SH人Gene SilencingPuromycinGene Silencing sc-92444-V人Gene SilencingPuromycinGene Silencing sc-155602小鼠 Gene SilencingN/AGene Silencing sc-155602-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155602-V小鼠 Gene SilencingPuromycinGene Silencing sc-155603小鼠 Gene SilencingN/AGene Silencing sc-155603-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155603-V小鼠 Gene SilencingPuromycinGene Silencing sc-155604小鼠 Gene SilencingN/AGene Silencing sc-155604-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155604-V小鼠 Gene SilencingPuromycinGene Silencing sc-155605小鼠 Gene SilencingN/AGene Silencing sc-155605-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155605-V小鼠 Gene SilencingPuromycinGene Silencing sc-155606小鼠 Gene SilencingN/AGene Silencing sc-155606-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155606-V小鼠 Gene SilencingPuromycinGene Silencing sc-155607小鼠 Gene SilencingN/AGene Silencing sc-155607-SH小鼠 Gene SilencingPuromycinGene Silencing sc-155607-V小鼠 Gene SilencingPuromycinGene Silencing
Zinc finger FYVE domain-containing proteins (ZFYVEs) are members of the FYVE-finger family of proteins. The FYVE domain is a cysteine-rich domain of about 70 amino acids that plays a role in the endosomal localization of the FYVE-finger proteins, and a majority of these proteins serve as regulators of endocytic membrane trafficking.