lambda100bp dna marker-CpoI 多少bp

来源：蜘蛛抓取(WebSpider) 时间：2016-09-02 14:25 标签： 10bp dna ladder
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&&FastDigest(R) SnaBI (Eco105I)
FastDigest(R) SnaBI (Eco105I)
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Reaction conditionsReaction temperatureDigestion time with 1ul of FastDigest(R) enzyme, minbp from end of DNA required for complete digestionThermal inactivationIncubation time without star activity, hoursLambda, 1ug/20ulPlasmid DNA, 1ug/20ulPCR product, ~0.2ug/30ulGenomic DNA, 1ug/10ul37°C5555365°C, 5 min16Lambda DNA0.7%琼脂糖1 切割位点配方1 ul (1 FDU)是指在37°C，1X FastDigest(R) 缓冲液中5分钟内酶切1 ug lambda DNA/CpoI片段所需的酶量。推荐反应条件1X FastDigest(R)缓冲液或 1X FastDigest(R) Green 缓冲液，37°C。1 ul FastDigest(R) SnaBI (Eco105I)可在– 5分钟内酶切1 ug lambda DNA/CpoI片段;– 5分钟内酶切1 ug质粒DNA;– 5分钟内酶切0.2 ug PCR扩增产物;– 5分钟内酶切1 ug基因组DNA或30分钟内酶切5 ug基因组DNA。商业化同裂酶采用REsearch(TM)软件寻找同功酶。甲基化影响Dam: 无重叠 – 不影响。Dcm: 无重叠 – 不影响。CpG: 完全重叠 – 阻止酶切。EcoKI: 无重叠 – 不影响。EcoBI: 无重叠 – 不影响。Methylation typeSequenceCleavage effectCpGTACGTA阻止酶切Number of recognition sites in DNA moleculesLambdaΦX174M13mp18/19pBR322puc18/19pUC57101000pTZ19R/UpTZ57RpBluescriptIIKS(-/+)pBluescriptIISK(-/+)pACYC177pACYC184000000
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