thermobi03da吧 fusca是什么菌

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Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
对Thermobifida fusca海藻糖合成酶(TreS)保守区域的氨基酸残基I224、N242、Q333、E352和N415进行定点突变,结果表明:位点I224、N242、N415突变后酶的活力与野生型TreS相近,E352位点突变后酶的比活力提高为野生型TreS的1.25倍.突变酶的最适温度、pH、Km和Kcat没有明显变化;突变Q333R则使TreS丧失了酶活力.
WANG Qing-yan
CHEN Fa-zhong
HUANG Fu-bao
WEI Chuan-dong
WEI Yu-tuo
HUANG Ri-bo
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广西大学,生命科学与技术学院,广西,南宁,530005
南宁中诺生物工程有限责任公司,广西,南宁,530003
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国家重点基础研究发展计划(973计划)
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, May 2010, Pages 121–127
Biochemical characterization of the cutinases from Thermobifida fusca, , , , , , , , , , , a State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave., Wuxi, Jiangsu 214122, Chinab School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Ave., Wuxi, Jiangsu 214122, Chinac Institute of Biochemistry, Department of Microbiology and Bioprocess Technology, University of Leipzig, Johannisallee 21-23, Leipzig D-04103, GermanyThermobifida fusca produces two cutinases which share 93% identity in amino acid sequence. In the present study, we investigated the detailed biochemical properties of T. fusca cutinases for the first time. For a better comparison between bacterial and fungal cutinases, recombinant Fusarium solani pisi cutinase was subjected to the similar analysis. The results showed that both bacterial and fungal cutinases are monomeric proteins in solution. The bacterial cutinases exhibited a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters. In addition, the two isoenzymes of T. fusca and the F. solani pisi cutinase are similar in substrate kinetics, the lack of interfacial activation, and metal ion requirements. However, the T. fusca cutinases showed higher stability in the presence of surfactants and organic solvents. Considering the versatile hydrolytic activity, good tolerance to surfactants, superior stability in organic solvents, and thermostability demonstrated by T. fusca cutinases, they may have promising applications in related industries.AbbreviationsFspC, Fusarium solani pisi cutinase; TDOC, sodium taurodeoxycholate; pNPB, p-nitrophenyl butyrate; pNPP, p-nitrophenyl palmitate; TPA, terephthalic acid; MHET, mono(2-hydroxyethyl terephthalate); BHET, bis(2-hydroxyethyl terephthalate); EMT, 1,2-ethylene-mono-terephthalate-mono(2-hydroxyethyl terephthalate)KeywordsThermobifida fusca; Cutinase; Characterization
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engineered thermobifida fusca cutinase with increased activity on polyester substrates
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engineered thermobifida fusca cutinase with increased activity on polyester substrates
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