您现在的位置：&&国外媒体评选电视游戏最性感女性角色TO5
　　国外网站近日以在电视游戏中登场的女性角色中最性感的五名角色为主题，选出了TOP5的最性感角色。不知各位玩家认同度如何呢。下面就是其评选出来的5名电视游戏最性感女性角色。
　　霞(Kasumi)-《死或生》游戏系列，网络游戏《生死格斗Online》
　　雾幻天神流的第18代首领，有一天，已经被逐出师门的霸神门的雷道(绫音的父亲，疾风与霞的叔叔)突然出现在忍者村中，并将霞的兄长打至重伤。为了揭开这一事件的真相，也为了替兄长报仇，霞不惜放弃了忍者首领的地位，参加了生与死大会。
　　虽然霞在第一届大赛中获得了冠军，但是她在大赛中所展现出来雾幻天神流的强大却给她以及她的兄长带来了灾难。在大会结束后，霞与疾风都被神秘组织“DOATEC”绑架，作为实验体进行研究。
　　春丽(Chun Li)-《街头霸王》系列
　　春丽是一位美丽漂亮的ICPO(国际刑警)特别调查员。为了替被妄图征服世界的邪恶头目维加所杀害父亲报仇，年仅18岁的她毅然决然地加入了刑警这个行业。温柔善良的她经常为了失踪的父亲而落泪。
　　春丽除了精通中国拳术和格斗技能外，还以第6位的成绩在国际警察射击大会上获奖，是个非常有实力的女性格斗家。她独立研究父亲传授的拳法，加入自己的理解，磨练出纯熟的跳跃技能，形成了独特的春丽格斗，其中最为突出的就是其脚力、平衡性以及掌气的运用。
　　不知火舞(Mai Shiranui)-《拳皇》系列
　　不知火舞是电视游戏《拳皇》系列中的女性角色之一。继承不知火流忍术的女忍者，因为喜欢上了在自己祖父不知火半藏门下修行的安迪伯格，在追随他的过程中卷入多场战斗。使用扇子等多种忍术进行攻击，战斗时的胸部晃动堪称最大的特色，和在游戏中给人留下的艳丽造型不同，设定里不知火舞是个标准的持家型好女友，温柔体贴，而且擅长料理，只是偶尔会对安迪的迟钝犹豫而大发脾气。
新闻点击排行榜Queen B声色诱惑 性感全释放- 1折网
> 购物经验 > Queen B声色诱惑 性感全释放
Queen B声色诱惑 性感全释放
莉顿&梅斯特 (Leighton Meester) 继在《Good Girls Go Bad》中&客串&之后，她终于推出了自己的首支单曲《Somebody to Love》，MV已经曝光，莉顿&梅斯特 (Leighton Meester) 在其中带来了大尺度的性感演出，真能让人一边狂惊艳一边狂喷血!
莉顿&梅斯特 (Leighton Meester)
&　　《Gossip Girl》莉顿&梅斯特 (Leighton Meester) 要进军歌坛的消息大家早就知道并且一直在热切期待啦，继在《Good Girls Go Bad》中&客串&之后，她终于推出了自己的首支单曲《Somebody to Love》，MV已经曝光，莉顿&梅斯特 (Leighton Meester) 在其中带来了大尺度的性感演出，真能让人一边狂惊艳一边狂喷血!
&　　除了带来性感MV《Somebody to Love》，最近莉顿&梅斯特 (Leighton Meester) 还为男性时尚杂志《GQ》12月号拍摄了一组性感大片，Queen B的双重&声色诱惑&你受得了吗?
莉顿&梅斯特 (Leighton Meester)
Queen B这回要准备把她的性感一次性释放到淋漓尽致了~
莉顿&梅斯特 (Leighton Meester)
莉顿&梅斯特 (Leighton Meester)
&　　关于Queen B的新专辑：
　　目前官方仍未公布莉顿&梅斯特 (Leighton Meester) 新专辑的发行日期，且专辑名称也处于保密阶段。有传闻称此次专辑将以她的名字命名&&《莉顿&梅斯特》。据莉顿&梅斯特 (Leighton Meester) 本人介绍，粉丝们透过这张个人大碟将看到的是她的人生快照。
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iframe(src='///ns.html?id=GTM-T947SH', height='0', width='0', style='display: visibility:')人上皮膜抗原(EMA)ELISA试剂盒
来源:上海研谨生物科技有限公司
浏览人数: 388
人上皮膜抗原(EMA)ELISA试剂盒
本试剂盒用于测定人血清，血浆及相关液体样本中上皮膜抗原(EMA)的含量。
人上皮膜抗原(EMA)人上皮膜抗原(EMA)抗体上皮膜抗原(EMA)HRP上皮膜抗原(EMA)--TMBTMBHRP上皮膜抗原(EMA)450nmOD人上皮膜抗原(EMA)的浓度。
1. 10-2020/
2. EDTA10-2020/
4. 20/PBSPH7.2-7.4100/ml20/
5. PBSPH7.42-8PBSPH7.420/
6. -20℃保存，但.
7. 不能检测含NaN3的样品NaN3HRP
10100&l50&l100&l50&l50&l50&l50ul50&l50&l50&l50&l50&l50&l300 pg/ml200 pg/ml100 pg/ml50 pg/ml25 pg/ml
37℃温育30
3048T203048T20
A50&lB50&l37℃避光显色15分钟.
450nmOD 15
021-& :&&&& 021-
&&&&&& E-mail&&&
FOR RESEARCH USE ONLY
Human &Epithelial membrane antigen
Drug Names
Generic Name：Human Epithelial membrane antigen (EMA) ELISA Kit.
This kit allows for the
in Human serum, blood plasma, and other.
Principle of the assay
The kit assay Human EMA level in the sampleuse Purified Human EMA to coat microtiter plate wells, make solid-phase antibody, then add EMA to wells, Combined EMA antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of
r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of
r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of
r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of
r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of
r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBSPH7.4, Homogenized by hand or Grinders, centrifugation 20-min at the speed of
r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can&t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can&t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100&l to the first and the second well, then add Standard dilution 50&l to the first and the second well, take out 100&l form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50&l to the third and the forth well , then take out 50&l from the third and the forth well discard, add 50&l to the fifth and the sixth well ,then add Standard dilution 50&l to the fifth and the sixth well, take out 50&l from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50&l to the seventh and the eighth well , take out 50&l from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50&l to the ninth and the tenth well, mix , take out 50&l from the ninth and the tenth well discard(add Sample 50&l to each well after Diluting ,(density: 300 pg/ml200 pg/ml100 pg/ml50 pg/ml25 pg/ml)
2.add sampleSet blank wells separately (blank comparison wells don&t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40&l to testing sample well, then add testing sample 10&l (sample final dilution is 5-fold), add sample to wells , don&t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washingUncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzymeAdd HRP-Conjugate reagent 50&l to each well, except &blank well.
7.incubateOperation with 3.
8.washingOperation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50&l to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（&n&5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
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