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应生物与环境工程系生物工程硕士建设点邀请，湖南师范大学邓云教授和华中农业大学陈天圣教授先后到我校为该系师生作学术报告，报告会由生物工程学科带头人张建设教授主持。
邓云教授的学术报告以&模式动物斑马鱼技术平台构建&为题，介绍了该技术平台的主要研究思路和技术路线、技术平台构建的主要方法和工作重难点，突出了斑马鱼作为模式生物在科学研究中的优势。报告会后，邓云教授还来到了生科苑1号楼，现场指导了斑马鱼繁养系统的使用方法，分享了实践操作经验。
邓云，博士，湖南师范大学生命科学学院教授，硕士导师。建立了湖南省唯一标准的斑马鱼模型技术平台。主持国家自然科学基金4项，省部级项目7项。在Cir Res，CMM等国际刊物发表SCI论文30余篇。2009年获湖南省自然科学一等奖；2010年成功入选湖南省121人才工程。
陈天圣教授的报告主要围绕鱼类干细胞研究思路和技术路线、细胞培养技术要点和转基因鱼类细胞系等方面开展，介绍了近年来取得的主要成果和研究经验。陈教授详细介绍了鱼类细胞培养的技术要点和重难点，分享了在试剂选购、样本取材、技术路线选择等方面的宝贵工作经验，并耐心细致地回答了在场师生的提问。
陈天圣博士是华中农业大学水产学院教授，博士生导师，主要从事鱼类发育与干细胞相关领域研究。在国际核心权威期刊发表研究论文十余篇，SCI他引300多次，以口头报告或会议摘要的形式参加国际学术会议二十多次。2010年实验中心发表论文情况 - 湖南师范大学生物学实验中心
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作者论文题目刊物 年、卷、期、页彭贤锦*Six new species of the spider genus Pardosa (Araneae: Lycosidae)
From Yunnan Province. China.Oriental Insect -404彭贤锦*Two new spider species of the genus Tetragnatha from yunnan Province, China
(Araneae, Tetragnathidae).Acta Arachnologica Sinica ),7-10彭贤锦*The orb-weaving spider genus Eriovixia (Araneae: Araneidae) in the
Gaoligong Mountains, China.Zootaxa -51彭贤锦*The spider genus Cnodalia (Araneae: Araneidae) in ChinaZootaxa -66常海艳, 陈则*Comparison of adjuvant efficacy of chitosan and aluminum hydroxide for
intraperitoneally administered inactivated influenza H5N1DNA Cell Biol ),563-8常海艳, 陈则*A single dose of DNA vaccine based on conserved H5N1 subtype proteins
provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1
influenza virus.Virol J 印大中*羰基应激与亚健康第四届国学国医岳麓论坛精选论文集 2010陈湘定Gene expression profiling in monocytes and SNP association
suggest the importance of STAT1 gene for osteoporosis in both Chinese and
Caucasians.J Bone Miner Res -355陈湘定Association study on bone mineral density with SNPs at the
regions of 2q24.3, 5q21, 7p21.2 and 13q21.1 in Chinese population国际骨质疏松基金会/中华医学会 2010陈湘定*中美人群乙醇脱氢酶基因单核苷酸多态性差异分析中南四省动物学会论文集 2010丛义艳, 刘克明*Impatiens oblongipetala (Balsaminaceae), a New Species from
Yunnan, ChinaNOVON ),392-395丛义艳, 相银龙, 刘克明*Impatiens quadriloba sp. nov. (Balsaminaceae) from Sichuan,
ChinaNordic Journal of Botany ),309-312戴国, 汪保和*AcSDKP对体外培养条件下人骨髓间充质干细胞增殖周期的影响湖南师范大学自然科学学报 ): 115-120邓乐两相体系中微生物转化制备依西美坦的研究湖南师范大学自然科学学报 ): 112-118邓乐链霉亲和素磁珠的合成及其用于AMP适配子传感器的研究生命科学研究 ),507-515邓云Synergistic efficacy of LBH and αB-crystallin through inhibiting
transcriptional activities of p53 and p21BMB reports ),432-437邓云ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits
AP-1- and SRE-mediated transcriptional activityBMB reports ),193-198邓云*SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and
p21-mediated transcriptional activityBMB reports ),355-361邓云*ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses
NFAT and p21 pathwayBMB reports ),212-218姜孝成*水稻种子脱水耐性的形成及其与贮藏特性的关系种子 ): 25-29黎维平横斜系（菊科紫菀属）的核型研究武汉植物学研究 ): 406-409李东屏*ANK6, a mitochondrial ankyrin repeat protein, isrequired for
male- female gamete recognition in Arabidopsis thalianaProc Natl Acad Sci USA ): 2印大中*Malondialdehyde suppresses cerebral function by breaking
homeostasis between excitation and inhibition in turtle Trachemys
scriptaPLoS ONE ),e1李国林,& 印大中*Direct reaction of taurine with malondialdehyde: evidence for
taurine as a scavenger of reactive carbonyl speciesRedox Report ): 268-274李国林*, ,印大中Age-related carbonyl stress and erythrocyte membrane protein
carbonylationClinical Hemorheology and Microcirculation ):
305-311李建中*鱼类促性腺激素受体研究进展生命科学研究 ): 263-267李建中*草鱼促性腺激素FSH和LH基因的克隆、特征及表达研究Fish Physiology and Biochemistry ): 213-221李选文, 陈平*, 梁宋平*Proteomics analysis of plasma membrane from liver sinusoidal
endothelial cells after partial hepatectomy by an improved two-dimensional
electrophoresis.Molecular and Cellular Biochemistry 7-150李烨, &印大中*PA28γ及其胞浆定位突变体在HepG2中的表达中国生物化学与分子生物学报 ): 369-373李永青*斑马鱼Lefty1特异性多克隆抗体的制备激光生物学报 ),685-689李永青*小鼠Lbh抗体的制备和鉴定激光生物学报 ),483-487梁满中超级杂交稻杂种优势基因组水平的转录分析中国农业杂志（学术版） ),71-75梁满中农垦58S及其衍生的光敏核不育系育性感光感温性遗传研究杂交水稻 2010梁满中Expression Analysis of miRNAs and Highly-expressed Small RNAs in
Two Rice Subspecies and their Reciprocal HybridsJournal of Integrative Plant Biology ),971–980梁满中*短光敏核不育水稻D38S育性的感光性和感温性研究杂交水稻 2010梁宋平Protocols for Peptidomic Analysis of Spider VenomsPeptidomic of Spider Venoms 梁宋平*Mechanism of action of two insect toxins huwentoxin-III and
hainantoxin-VI on voltage-gated sodium channelsBiomedicine & Biotechnology) ),451-457梁宋平*,曹锐Improvement of hydrophobic integral membrane protein
identification by mild performic acid oxidation-assisted digestionAnalytical Biochemistry ),196-204梁宋平*ATDB 2.0: A database integrated toxin-ion channel interaction
dataToxicon -677梁宋平*Evaluation and optimization of removal of an acid-insoluble
surfactant for shotgun analysis of membrane proteomeElectrophoresis 05-2713梁宋平*, 陈平*Shotgun Proteomics and Network Analysis Between Plasma Membrane
and Extracellular Matrix Proteins From Rat Olfactory Ensheathing
CellsCell Transplantation, ): 133-146梁宋平*The Tarantula Toxins ProTx-II and Huwentoxin-IV Differentially
Interact with Human Nav1.7 Voltage Sensors to Inhibit Channel Activation and
InactivationMol &Pharmacol ): 梁宋平*TranscriptomeanalysisofthevenomglandsoftheChinesewolf spider
LycosasingoriensisZoology ),10-18梁宋平*,Dried polyacrylamide gel absorption: A method for efficient
elimination of the interferences from SDS-solubilized protein samples in mass
spectrometry-based proteome analysisElectrophoresis -7梁宋平*,Gel absorption-based sample preparation for the analysis of
membrane proteomeAnalytical Biochemistry -210刘克明*Impatiens quadriloba sp. nov. (Balsaminaceae) from Sichuan,
ChinaNordic Journal of Botany ),309-312刘少军*Microsatellite analysis of different ploidy offspring of
artificial gynogenesis in C.carpio (Cyprinus carpio L.)Journal of Fish Biology 2010, DOI: 10.1111/j. .
.x刘少军*金鱼生殖细胞多倍化现象分析水产学报 ): 刘少军*Evidence for the Formation of the Male Gynogenetic
FishMarine Biotechnology 0-172刘少军*Identification of genes involved in the early ovary development
of diploid gynogen populations from allotetraploid hybrids using suppression
subtractive hybridization.Marine Biotechnology 6-194刘少军*转生长激素基因三倍体鲤鱼的快速生长与不育特性科学通报 ): 刘少军*Analysis of 5S rDNA Organization and Variation in Polyploid
Hybrids from Crosses of Different Fish Subfamilies.J. Exp. Zool. (Part B: Mol. Dev. Evol.) 3–411刘少军*Comparative analysis of mitochondrial control region in
polyploid hybrids of red crucian carp (Carassius auratus) × blunt snout bream
(Megalobrama amblycephala)Fish Physiology and Biochemistry 3-272刘少军*Isolation and expression analyses of the Sox9a gene in triploid
crucian carpFish Physiology and Biochemistry 5-133印大中*浅析中医调养的内在机理第四届国学国医岳麓论坛精选论文集 2010刘文彬, 李万程*PP-1与PP-2A在金鱼眼(球)不同组织/细胞中的分化表达模式中国生物化学与分子生物学报 2010,(12): 陈平*, 梁宋平*Shotgun proteomics and network analysis between plasma membrane
and extracellular matrix proteins from rat olfactory ensheathing
cells.Cell transplation. ): 133-146莫小阳A new species of Megophrys (Amphibia: Anura: Megophryidae) from
the northwestern Hunan Province, China.Current Zoology ): 431-435莫小阳*MVP interacts with YPEL4 and inhibits YPEL4-mediated activities
of the ERK signal pathwayBiochem Cell Biol ): 445-50唐果, Theo Blick, Hirotsugu Ono*Rediscovery of an obscure spider genus Zametopina Simon, 1909
(Araneae, Thomisidae) from Yunnan, ChinaBulletin of the National Science Museum, Tokyo, (A)
),65-70万永奇*Synergistic efficacy of LBH and alphaB-crystallin through
inhibiting transcriptional activities of p53 and p21.BMB Rep. ),432-437陈则*糖尿病小鼠对H5N1病毒的易感性及灭活疫苗诱导的免疫反应生命科学研究 5-339王贤纯*Dried polyacrylamide gel absorption: A method for efficient
elimination of the interferencesfrom SDS-solubilized protein samples in mass
spectrometry-based proteome analysis.Electrophoresis 16-3822王贤纯*Evaluation and optimization of removal of an acid-insoluble
surfactant forshotgun analysis of membrane proteome.Electrophoresis 05-2713王跃群*Nodal 蛋白的表达、纯化及多克隆抗体制备激光生物学报 ),813-816王跃群*KLHL31促进C2C12细胞的肌原分化激光生物学报 ),466-468王跃群*Nulp1基因抑制P19CL6细胞向心肌细胞分化激光生物学报 ),246-248王跃群*小鼠RMND5A的原核表达、纯化及多克隆抗体制备激光生物学报 ),273-277肖玲, 周建林*Identification of a novel human cancer/testis gene MAEL that is
regulated by DNA methylationMolecular Biology Reports ),肖亚梅Contrast Expression Patterns of JNK1 during Sex Reversal of the
Rice-field EelJ.EXP.Zool(Mol.Dev.Evol.) 4B): , 242-256肖亚梅Molecular clone of the genes encoding the PR55/B regulatory
subunits for PP-2A and analysia of their functions in regulating development of
Goldfish, Carassius auratusGene regulation and systems biology -14谢伏霞, 印大中*Aging resolution and perspectives2010 SICBM Book 2010李国林, 印大中*以尿液毒性羰基物质测评亚健康状态的简捷方法湖南师范大学自然科学学报 ),121-126徐孟亮*一个反向水稻两用核不育系育性对温度与光周期的反应湖南师范大学自然科学学报 ),85-88徐孟亮*茶陵野生稻苗期耐冷性与抗氧化系统的关系生命科学研究 ),67-72徐孟亮*几个新选水稻两用核不育系育性对光周期与温度的反应湖南师范大学自然科学学报 ),88-91印大中前言-能量代谢应激与衰老生化本质实用老年医学 )印大中羰基应激与衰老生化老年药理学与药物治疗学 2010印大中*熵增与衰老中华医学会老年医学分会衰老及老年相关疾病的临床与基础研究学术会议 2010袁婺洲*myh6蛋白的表达、纯化及多克隆抗体制备激光生物学报 4-647袁婺洲*ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits
AP-1- and SRE-mediated transcriptional activityBMB reports ),193-198袁婺洲*利用果蝇心力衰竭模型筛选果蝇第2号染色体缺失系生命科学研究 ),195-198袁婺洲*斑马鱼心脏标记基因pitx2的多克隆抗体的制备湖南师范大学自然科学学报 ),98-101曾雄智*敬钊毒素-V对Kv4.3通道的抑制作用生理学报 ),255－260张纯金鱼生殖细胞多倍化现象分析水产学报 ),张健*CK2 phosphorylates TNFAIP1 to affect its subcellular
localization and interaction with PCNAMolecular Biology Reports ),2967-73张健*Aspirin inhibits ErbB2 to induce apoptosis in cervical cancer
cellsMed Oncol ),379-387张健*Functional characterization of the promoter region of human
TNFAIP1 geneMolecular Biology Reports ),刘文彬, 肖亚梅, 李万程*Molecular Cloning of the Genes Encoding the PR55/Bβ/δRegulatory
Subunits for PP-2A and Analysis of Their Functions in Regulating Development of
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modelJ of Genetics and Genomics 3-603周琼*昆虫肠组织石蜡切片的制作华中昆虫研究 2010, 6周琼*农药对蜘蛛的影响华中昆虫研究 2010, &6周琼*蜘蛛捕食行为和化学通讯的研究华中昆虫研究 2010, 6
此篇来自：湖南师范大学生命科学学院精品课程_土豆_高清视频在线观看心脏特异表达绿色荧光斑马鱼模型的建立与评估
Vol 35, No.4
& 核心刊物 & 遗传
Vol 35, No.4 & 心脏特异表达绿色荧光斑马鱼模型的建立与评估
心脏特异表达绿色荧光斑马鱼模型的建立与评估
彭夕洋, 陈婷芳, 黄婷, 江志钢, 吴秀山, 邓云
湖南师范大学心脏发育研究中心, 发育生物学与蛋白质组学教育部重点实验室, 长沙 410081
Key Words：& 斑马鱼
Tol2 转座子
本课题组前期研究中, 利用斑马鱼cmlc2 (Cardiac myosin light chain 2)基因启动子构建了一个用于斑马鱼心脏组织特异表达外源基因的转基因表达载体pTol2-cmlc2-IRES-EGFP。文章利用该载体构建了一个稳定表达EGFP的转基因斑马鱼品系, 并初步分析了EGFP的表达对该转基因斑马鱼品系的心脏发育和功能的影响。结果表明, 在建立的转基因斑马鱼品系早期胚胎发育过程中, 绿色荧光信号在心脏中特异表达, 该表达模式与原位杂交分析的cmlc2的表达模式结果相同; 该转基因斑马鱼品系的心脏形态及发育生长正常; 进一步通过M-Mode分析心脏生理学功能的结果表明：该转基因品系心动周期、心率、收缩与舒张表面积及表面积缩短率等重要生理指标与正常野生型的斑马鱼对照组相比没有显著差别。以上结果表明该转基因品系中绿色荧光蛋白的表达对斑马鱼心脏的发育和功能没有影响。研究结果为进一步利用该载体建立外源目的基因转基因表达模型, 研究心脏表达基因的功能奠定了重要基础。
Using the promoter for cardiac myosin light chain 2 (cmlc2) gene, an expression vector pTol2-cmlc2-IRES-EGFP for making heart-specific expression of exogenous gene in transgenic zebrafish was generated previously. Here, we reported the construction of a transgenic zebrafish line which stably expresses EGFP using this vector, and the effects of EGFP on the heart development and cardiac function of this transgenic zebrafish line were preliminarily analyzed. The results showed that the green fluorescence signal of cmlc2:EGFP line under fluo-rescence microscopy specifically expressed in heart and faithfully recapitulated both the spatial and temporal expression patterns of endogenous cmlc2 gene revealed by in situ hybridization in the early developmental stages. The cardiac morphology and development of this transgenic zebrafish line remained to be normal. Furthermore, the heart mor-phology and physiological function of this transgenic line have been analyzed using M-mode analysis. The results showed that there was no significant difference between the cmlc2:EGFP and the wild type lines with respect to heart period, heart rate, diastolic surface area and systolic surface area, and fractional area change. No tachyarrhythmia was observed in the embryos from either line. Thus, the excessive expression of EGFP in this transgenic line seemed to exert no detrimental effects on the function and development of zebrfish hearts during early stages. Our study laid a foundation for the construc-tion of exogenous gene transgenic line using pTol2-cmlc2-IRES-EGFP vector to study the function of genes that expressed in heart.
编辑单位:《遗传》 编辑部
主 编: 张永清
主 任: 张颖
：审稿专家
国内刊号：CN11-1913/R
国际刊号：ISSN
邮发代号： 2－810