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《Disruption of chemotaxisrelated genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi》.pdf 6页
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Ocean01.Sin.,2013,V01.32,No.8,R55.60
DOI:10.1007/s1—0341-1
http://www.hyxb.org.cn
E—mail:hyxbe@263.net
ofchemotaxis-relatedaffects
Disruption
genes multiple
andthevirulenceof
pathogenicharveyi
361021,China
2Fisheries
361021,China
College,IimeiUniversity,Xiamen
ofOceanandEarth
Science,Xiamen
361005,China
University,Xiamen
7December2012
April2012;accepted
@TheChineseSocietyofOceanographyand
Springer—VerlagBerlinHeidelber92013
Chemotacticisinvolvedinthevirulenceof
bacteria.Inordertounderstand
manypathogenic
therole chemotacticofVibrio
incellular
processesvirulence.mini一砌j0/Kan
mutantswithdeficientchemotacticwere
transposon.induced
motilityconstructed,screened.and
revealedthatthe
accessionnumber
tiffed.Sequenceanalysis
465一bpfragment(GenBankHM630274)flank—
insertionsiteinmutantTS—CMlhadthe
ing transposon
highestidentity(96.9%)withhyoothetical
BAA一1116andthe
proteingene harveyiATCC
second—highestidentity(91.8%)withpgkgene
RIMD2210633.Inanothermutant。TS—CM2.356of
parahaemolyticus
bp transposon—flankingsequence
accessionnumber
HM630275)alsohighestidentity(94.6%)withhypotheticalpro—
ATCCBAA一1l16andthe
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